Josh Allen has run for a 26-yard touchdown on fourth down with 2:17 left, sealing the Buffalo Bills' 30-21 win over the Kansas City Chiefs and handing the two-time defending Super Bowl champions their first loss of the season.

James Cook rushed for two touchdowns and Allen threw a 12-yard TD pass to Curtis Samuel for Buffalo (9-2), who closed the gap on Kansas City (9-1) in the race for the AFC's top seed.

Patrick Mahomes' Chiefs were the NFL's last undefeated team and finally ran out of ways to eke out one-score victories and overcome second-half deficits. The Chiefs had won 15 straight, including playoffs, since they fell 20-14 to the Las Vegas Raiders on December 17, 2023.

The Bills have won six straight and moved closer to their sixth straight AFC East title.

Buffalo have defeated the Chiefs in four straight regular-season meetings, though Kansas City hold the post-season edge, having won the past three match-ups dating to 2020.

Bills coach Sean McDermott opted to go for it on fourth-and-two instead of kicking a field goal for a five-point lead. Allen faked a pass to his left and then broke up the middle, where he bounced off two Chiefs defenders before crossing the goal line.

Allen finished 27 of 40 for 262 yards with the TDs passing and rushing and an interception.

The rushing touchdown was the 58th of his career, moving him into second on the Bills list, one ahead of O.J. Simpson, and seven behind Thurman Thomas. Allen's two-touchdown outing also increased his total to 244, tying Jim Kelly for first in team history.

"When Josh takes off, I find myself just watching, like, 'Wow, he's different,'" Bills receiver Khalil Shakir said.

"When he took off today, I just started yelling. I couldn't stop yelling. I was blanking in the head."

Mahomes finished 23 of 33 for 196 yards with three touchdown passes ? two to tight end Noah Gray and another to Xavier Worthy ? and two interceptions.

"The undefeated thing was cool. But that's not our ultimate goal," Mahomes said.

"That's a good football team. Nothing to hang your head on losing to them."

Elsewhere, Jared Goff threw four touchdown passes and Detroit routed Jacksonville 52-6.

The NFC-leading Lions (9-1) have won eight straight and nine of their first 10 games in a season for the first time since 1934.

Detroit's winning margin of 46 points was the largest in franchise history, and it handed the Jaguars (2-9) their biggest-ever defeat.

There were also home wins for the Los Angeles Chargers, Miami, New Orleans, Pittsburgh and Denver, while Green Bay, the LA Rams, Indianapolis, Minnesota and Seattle triumphed on their travels.

With AAP.